應用脂肪測定儀準確測定桔梗中的皂苷含量
桔梗宣肺、利咽、祛痰、painongzhigongxiao。linchuangchangyongyukesoutanduo,xiongmenbuchang,yanzhong,yinya,feiyongtutan,chuangyangnongchengbukuidengzheng。jugengdezhiliangpingjiadezhuyaozhibiaoshitongguozongzaoganhanliang,takeyishiyongshiyong粗脂肪測定儀來(lai)進(jin)行(xing)測(ce)定(ding),在(zai)以(yi)前(qian)經(jing)常(chang)使(shi)用(yong)傳(chuan)統(tong)的(de)索(suo)氏(shi)提(ti)取(qu)器(qi)法(fa)進(jin)行(xing)提(ti)取(qu)測(ce)定(ding)。但(dan)是(shi)在(zai)使(shi)用(yong)這(zhe)種(zhong)方(fang)法(fa)進(jin)行(xing)測(ce)定(ding)的(de)時(shi)候(hou)發(fa)現(xian),利(li)用(yong)這(zhe)一(yi)方(fang)法(fa)進(jin)行(xing)桔(ju)梗(geng)皂(zao)苷(gan)含(han)量(liang)測(ce)定(ding)費(fei)工(gong)費(fei)時(shi)、效率低,單個樣品耗費藥品量大,難以同時進行批量提取處理。而使用脂肪測定儀就可以避免這種方法的缺點,下麵就來進行了解一下這種方法的操作步驟:
取桔梗樣品粗粉約4 g,精密稱定,置於抽提筒吊籃內,加50 mL甲醇,浸泡15 h。將抽提筒內吊籃提升5 cm,同時將冷凝管調節旋塞開啟,加熱回流6 h,抽提物放置過夜。濾過,關閉冷凝器調節旋塞,加熱濾液濃縮至15~20mL,放冷,加乙醚50 mL,振搖,放置至澄明,棄去上清液,沉澱分次加甲醇(20 mL,10 mL,5mL)加熱使溶解,放冷,濾過,合並甲醇液,濾液濃縮至15~20 mL,放冷,加乙醚50 mL,振搖,同上法處理,合並甲醇液,置已恒重的蒸發皿中,於水浴鍋上蒸發至幹,在105℃幹燥至恒重,計算,即得桔梗總皂苷。
shiyongzhezhongfangfajinxingcedingdeshihouzhunquedugao,tongshihuishoulvbishiyongsuoshitiqufalaidehaoyixie,caiyongzhifangcedingyitiqufazaihuishourongjishibimianleshiyanguochengzhonghuifayouduxingqiti,yijiyingxiangshiyanwucha,keyifangbiandijinxingnongsuodetongshihuishourongji。shiyanbiaoming,meicimeigeyangpinkeyihuishoujiachun50~75 mL,每次可同時測定6個樣品,共回收甲醇約300~450 mL。



